Microbiology, Immunology & Molecular Genetics Adjunct Faculty
Anthony Griffiths, Ph.D.
Texas Biomedical Research Institute
Department of Virology and Immunology
Southwest National Primate Rearch Center
(UTHSCSA School of Medicine, Microbiology, Immunology & Molecular Genetics)
Tel: (210) 258-9557
Fax: (210) 258-3329
EducationPh.D., University of Cambridge (Queens’ College)
Postdoctoral Training: Harvard Medical School
The family Filoviridae includes Ebola virus and Marburg virus, which are associated with sporadic outbreaks and high case fatality rates. A maximum containment laboratory (Biosafety Level-4) is required to study the infectious forms of these viruses. Dr. Griffiths' laboratory is interested in multiple aspects of filovirus biology with a view to exploiting this knowledge to further the development of vaccines and therapeutics.
Typically, RNA viruses have high spontaneous mutation rates due to error prone RNA-dependent RNA polymerases. The consequences of high spontaneous mutation and replication rates are populations composed of heterogeneous swarms of related variant sequences, sometimes called quasispecies. Dr. Griffiths's lab is investigating the importance of this diversity to filovirus replication and pathogenesis in vitro and in vivo and are particularly interested in exploiting the mutation rate as a therapeutic mechanism. Specifically, they work to understand the roles of the individual genotype populations in virus infection; for example, in any Ebola virus population there is a mixture of genotypes that appear to control the expression of different forms of the virus glycoprotein (GP). The ratios of the genotypes is dynamic and changes dependent on the origin species of cells used to propagate virus, and even in an infected animal. These changes are certainly important for the development of vaccines and therapeutic – that require animal models – but also for viral pathogenesis.
Many of these studies exploit recent advances in sequencing. They have several Illumina sequencing machines on our campus and a miSeq in the laboratory. This system permits quantification of the individual viral genotypes in a sample and they have developed techniques to rapidly sequence whole vira genomes, including the 5’ and 3’ termini. They are expanding the use of these machines into other areas including gene expression and ribosome profiling of cells and tissues infected with BSL-4 pathogens.
- Society for General Microbiology
- RNA Society
- American Society for Virology
- American Society for Microbiology
Qing Fan, Melanie Amen, Mallory Harden, Alberto Severini, Anthony Griffiths, and Richard Longnecker. (2012) “Herpes B virus and Cercopithecine herpesvirus 2 utilize human nectin-1 for entry, but not HVEM”. Journal of Virology. 86(8) 4468-4476. PMCID: PMC 3318624
- Amy C. Shurtleff, Julia E. Biggins, Ashley E. Keeney, Elizabeth E. Zumbrun, Holly A. Bloomfield, Ana Kuehne, Jennifer L. Audet, Kendra J. Alfson, Anthony Griffiths, Gene G. Olinger, Sina Bavari, and the Plaque Assay Standardization Subcommittee of the Filovirus Animal Nonclinical Group. (2012) Refinement and standardization of the filovirus plaque assay for use in preclinical studies. Viruses. 4(12), 3511-3530.
- Amy C. Shurtleff, Nicole Garza, Matthew Lackemeyer, Ricardo Carrion, Jr., Anthony Griffiths, Jean Patterson, Samuel S. Edwin, and Sina Bavari. (2012) The Impact of Regulations, Safety Considerations and Physical Limitations on Research Progress at High Biocontainment. Viruses. 4(12), 3932-3951
- Alfson KJ, Beadles MW, and A. Griffiths*. (2014) A new approach to determining whole viral genomic sequences including termini using a single deep sequencing run. Journal of Virological Methods. 208 (1), 1-5.
- Jens H. Kuhn *, Kristian G. Andersen, Yīmíng Bào , Sina Bavari, Stephan Becker, Richard S. Bennett, Nicholas H. Bergman, Olga Blinkova, Steven Bradfute, J. Rodney Brister, Alexander Bukreyev, Kartik Chandran, Alexander A. Chepurnov, Robert A. Davey, Ralf G. Dietzgen, Norman A. Doggett, Olga Dolnik, John M. Dye, Sven Enterlein, Paul W. Fenimore, Pierre Formenty, Alexander N. Freiberg, Stephen Gire, Jean-Paul Gonzalez, Anthony Griffiths, Robert F. Garry, Nicole L. Garza, Christian T. Happi, Lisa E. Hensley, Andrew S. Herbert, Michael C. Hevey, Thomas Hoenen, Anna N. Honko, Georgy M. Ignatyev, Peter B. Jahrling, Joshua C. Johnson, Karl M. Johnson, Jason Kindrachuk, Hans-Dieter Klenk, Gary Kobinger, Tadeusz J. Kochel, Matthew G. Lackemeyer, Daniel F. Lackner, Eric M. Leroy, Mark S. Lever, Elke Mühlberger, Sergey V. Netesov, Gene G. Olinger, Sunday A. Omilabu, Gustavo Palacios, Rekha HG. Panchal, Daniel J. Park, Jean L. Patterson, Janusz Paweska, Clarence J. Peters, James Pettitt, Louise Pitt, Sheli R. Radoshitzky, Elena I. Ryabchikova, Erica Ollmann blb, Erica Ollmann Saphire, Pardis C. Sabeti, Rachel Sealfon, Aleksandr M. Shestopalov , Sophie J. Smither, Nancy J. Sullivan, Robert Swanepoel, Ayato Takada, Jonathan S. Towner, Guido van der Groen, Viktor E. Volchkov, Valentina A. Volchkova, Victoria Wahl-Jensen , Travis K. Warren, Kelly L. Warfield, Manfred Weidmann, Stuart T. Nichol * Filovirus RefSeq Entries: Evaluation and Selection of Filovirus Type Variants, Type Sequences, and Names. Viruses (2014)
- Lee, Hughes, Rostal, Sitam, Lee, Japning, Harden, Griffiths, Basir, Wolfe, Epstein, and Daszak. (2015) Macacine Herpes virus 1 in Malaysian Free-ranging Long-tailed Macaques (Macaca fascicularis). Emerging Infectious Diseases. In press
- Alfson, Beadles, Menzie, Patterson, Carrion, and Griffiths*. (2015) Genetic Changes at the Glycoprotein Editing Site Associated with Serial Passage of Sudan Virus. J Infect Dis 2015; pii: jiv216 [Epub ahead of print]. PMCID: in process
- Alfson, Avena, Beadles, Staples, Nunneley, Ticer, Dick, Owston, Reed, Patterson, Carrion, and Griffiths*. Specific infectivity if Ebola virus impacts virulence in Macaca fasicularis. J Virol 2015; 89:6773-6781. PMCID: PMCID: PMC4468478 [Available on 2016-01-01].
- Cai, Parks, Wall, Stott, Stambaugh, Alfson, Griffiths, Mathies, Carrion, Patterson, Hawking, and Schmidt. Diagnosis of Ebola virus infection via amplification-free direct detection of single nucleic acids on a chip. Accepted for publication in Scientific Reports.
- Colgrove, Liu, Griffiths, Raja, DeLuca, Newman, Coen, and Knipe. History and genomic sequence analysis of the herpes simplex virus 1 KOS and KOS1.1 sub-strain. Accepted for publication in Virology.