Tel: (210) 567-6156
My initial studies involved quantifying the direct interaction of all seven 14-3-3 adaptors (seven homologous isoforms, 14-3-3β, 14-3-3ε, 14-3-3γ, 14-3-3η, 14-3-3σ, 14-3-3τ and 14-3-3ζ) with class switch DNA recombination (CSR) factors, including activation-induced cytidine deaminase (AID, encoded by the Aicda gene), both the catalytic and regulatory subunits of protein kinase A (PKA-Cα and PKA-RIα) and uracil DNA glycosylase (Ung). I found that disrupting the interaction between 14-3-3 and AID with the naturally occurring HIV-1 viral protein R (Vpr) and Vpr peptides, an accessory protein of human immunodeficiency virus type-1 (HIV-1), inhibited CSR by disrupting 14-3-3 and AID recruitment to immunoglobulin heavy chain (IgH) switch (S) region, suggesting that naturally occurring proteins or small molecules serve as potential therapeutics to inhibit unwanted CSR.
Understanding CSR mechanisms is crucial for the development of therapeutic enhancers of vaccines, and the development of therapeutic inhibitors of the generation of IgG and IgA autoantibodies that mediate tissue damage in autoimmune diseases (such as systemic lupus erythematosus) as well as IgE antibodies that mediate allergic responses (such as asthma).
I am highly encouraged by my novel findings showing a critical B cell-intrinsic role of an autophagic protein in the generation of class switched antibodies. I want to define the role of Rab7 in the molecular mechanisms underlying antibody class switching to IgG, IgA or IgE, for the development of therapeutics to inhibit class switching in a variety of clinical conditions. My doctoral studies would help to identify potential targets for future therapeutics to treat patients with autoimmune disease, allergy or B cell lymphoma.
American Association of Immunologists Trainee Abstract Award
American Association of Immunologists
March 2014, Travel award for the 2014 AAI Meeting in Pittsburgh, Pennsylvania.
NIH NIAID Immunology Research Training Grant T32 AI 60573
National Institute of Allergy and Infectious Diseases
2013-2014, One year training grant to support my research focused on the maturation of the antibody and autoantibody responses.
Science, Mathematics, And Research for Transformation Scholarship
Institute for Immunology, School of Biological Sciences, University of California Irvine, Irvine, California, United States of America
Scaffold Functions of 14-3-3 Adaptors in B Cell Immunoglobulin Class Switch DNA Recombination
PLOS ONE, November 25, 2013
Combinatorial H3K9acS10ph Histone Modification in IgH Locus S Regions Targets 14-3-3 Adaptors and AID to Specify Antibody Class-Switch DNA Recombination
Cell Reports, November 14, 2013
Induction of Activation-Induced Cytidine Deaminase–Targeting Adaptor 14-3-3γ Is Mediated by NF-κB–Dependent Recruitment of CFP1 to the 5′-CpG-3′–Rich 14-3-3γ Promoter and Is Sustained by E2A
Journal of Immunology, July 12, 2013
Drawing, jewelry design and music