Department of Cellular and Structural Biology

Optical Imaging Facility Instrumentation

 

Please consult with facility staff prior to anticipated usage, per HOP.

 

Confocal / Multiphoton Microscopy
System 1 Olympus FV-1000
Laser Scanning Confocal Microscopy
Room 2.518U Olympus IX-81 Microscope

All current and new users are required to read and acknowledge the new
Laser Standard Operating Procedures (SOP).
Olympus FV1000 Laser SOP

Objectives: Lasers:
  • 10X 0.40 NA (UPlanSApo, DIC)
  • 20X 0.75 NA (UPlanApo, DIC)
  • 25X 1.05 NA (XLPlan N, Water)-
    Available upon special request
  • 40X 0.60 NA (LUCPlanFLN, DIC)
  • 40X 1.30 NA (UPlanFLN, Oil, DIC)
  • 40X 1.00 NA (UPlanApoN, Oil, DIC)
  • 60X 1.42 NA (PlanApoN, Oil, DIC)
  • 60X 1.10 NA (LUMFI, Water) - Available upon request
  • Blue Diode (405nm)
  • Argon (458nm, 488nm, 515nm)
  • Green HeNe (543nm)
  • Red Diode (635nm)
  • Chameleon Multiphoton tunable laser, 715nm - 900nm

  • Spectral scanning
    3 Channel Detection for Fluorescence
    Brightfield Detection of DIC images
    Z-stepper motor
    Fluoview and Tiempo Ratio Imaging Software
    LSM Technology Objective Inverter
    Non-descanned external detector

     

    System 2 Zeiss 510 NLO
    (Confocal and Multiphoton)
    Room 2.518U Zeiss Axiovert 200 Microscope

    All current and new users are required to read and acknowledge the new
    Laser Standard Operating Procedures (SOP).
    Zeiss Laser SOP

    Objectives: Lasers:
  • 10X 0.30 NA (Plan-NEOFLUAR)
  • 40X 1.30 NA (Plan-NEOFLUAR, Oil, DIC)
  • 63X 1.40 NA (Plan-ApoCHROMAT, Oil, DIC)
  • 25X - Available upon request
  • 40X Water Immersion - Available upon request
  • 63X Water Immersion - Available upon request
  • 100X Oil - Available upon request
  • Argon (458/488/514 nm; 25 mW)
  • Green HeNe (543nm; 1 mW)
  • Red HeNe (633nm; 5 mW)
  • Coherent MIRA/5W VERDI fs TiS for multiphoton excitation imaging

  • Non-descanned Detection Single Channel for MP detection
    Z-stepper Motor
    Physiology Software
    LSM Flexible Scan Package
    3D Image Analysis
    Becker-Hickl SPC-730 FLIM detector (NDD) and analysis software
    LSM Technology objective inverter
    Programmable X-Y stage

     

    System 3 Nikon N-Storm Super Resolution Microscope STRF Room 252

    All current and new users are required to read and acknowledge the new
    Laser Standard Operating Procedures (SOP).
    Nikon N-Storm Laser SOP

    Equipped with Elipse Ti inverted TIRF microscope, and two high resolution CCD cameras: Photometrics Cool SNAP HQ2 and Andor iXon3 EMCCD.

    Objectives: Lasers:
  • CFI PLAN APO 10X NA 0.45
  • CFI Plan APO 20X NA 0.75
  • CFI Super Fluor 40X Oil NA 1.3
  • CFI APO 100X Oil TIRF NA 1.49
  • Violet diode laser: 405 nm
  • Green diode laser: 561 nm
  • Argon laser: 457, 477, 488, and 514 nm
  • Red fiber laser: 647 nm

  •  

    System 4 Nikon Swept Field Confocal Microscope with Photoactivation STRF Room 252

    All current and new users are required to read and acknowledge the new
    Laser Standard Operating Procedures (SOP).
    Nikon Swept Field Confocal SOP

    Equipped with Nikon Eclipse Ti inverted microscope and a Quant EM 512SC CCD camera. The slit and multi-pinhole scanning allows for rapid imaging.

    Objectives: Lasers:
  • CFI Plan Apo Lambda 10X NA 0.45
  • CFI Plan APO VC 20X NA 0.75
  • CFI Plan Fluor 40X Oil NA1.30
  • CFI Plan APO VC 60X Oil NA 1.4
  • CFI Plan APO VC 100X Oil NA 1.4
  • Violet diode laser 405 nm
  • Blue diode laser 488 nm
  • Green diode laser 561 nm
  • Red diode laser 640 nm

  •  

    System 5 Prairie Confocal/Two-Photon System STRF Room 252

    All current and new users are required to read and acknowledge the new
    Laser Standard Operating Procedures (SOP).
    Prairie Confocal SOP

    Equipped with Nikon Eclipse SN1 upright microscope and acoustic optical deflection (AOD) mode, which allows for video rate two-photon imaging.

    Objectives: Lasers:
  • 25X APO WI NA 1.10 (high NA and LWD)
  • Violet diode laser 405 nm
  • Blue diode laser 488 nm
  • Green diode laser 542 nm
  • Green diode laser 561 nm
  • Red diode laser 640 nm
  • Ti:Sapphire laser with a tunable range 750-900 nm

  •  

     

    Widefield Microscopy
    System 1 Nikon Eclipse 2000 Room 2.518U

    Objectives:
  • 10X 0.45 NA (PlanApo, DIC)
  • 20X 0.75 NA (PlanApo, DIC)
  • 40X 1.30 NA (S Fluor, Oil)
  • 60X (PlanApo TIRF, Oil, DIC)

  • Photometric CoolSnap HQ2 Camera
    NIS Elements Software
    FURA with Ratiometric Analysis
    Fluorescent filters (DAPI, FITC/GFP, Rhodamine, CY5) and DIC

     

    Fluorescence Stereo Microscopy
    System 1 Leica MZ16 FA Fluorescence Stereomicroscope Room 2.664U 16:1 zoom,
    Basic magnification
    Range 7.1x to 115x
    FluoCombi attachment

    Objectives:
  • 0.5X
  • 1.0X
  • 4X-10x

  • Fluorescence Filters: CFP, GFP, YFP, DsRed
    Transmitted, Fluorescence, Goose-neck light sources
    Image-ProPlus Analytical Imaging Software
    3D-Topography Analysis Software
    In-Focus Automation Software
    Hamamatsu Orca-AG camera
    CRI LCD Filter
    Chromophore Imaging

     

    Whole Animal Imaging (Bioluminescence, Fluorescence)
    System 1 IVIS Spectrum Room 1.389U (LAR)
    Fluorescence Filters Excitation - 10 (415 nm -760 nm (30 nm bandwidth each))
    Emission - 18 (490 nm -850 nm (20 nm bandwidth each))
    Quantum Efficiency >85% 500 - 700 nm
    >30% 400 - 900nm
    Lens f/1 - f/8
    Field of View (FOV) Minimum FOV - 3.9 x 3.9 cm
    Maximum FOV - 23 x 23 cm (up to 5 mice)
    Minimum Detectable Radiance 70 photons/s/cm2/sr
    Stage Temperature 20 - 40°C
    Transillumination Stage
    Isoflurane Gas Anesthesia external chamber, internal nose cones
    Dimensions Imaging Chamber Interior Size: 43Wx50Dx60H cm (17 x 20 x 24 in)
    Plate reader insert

     

    Ancillary Equipment
    Isofluorane anesthetizing machine
    Mini-vent ventilator
    Mouse Ox dosimeter
    CO2 Incubator
    Bioptechs stage incubator chambers
    Imaging system diagnostic equipment
    LSM Technologies Objective Inverters
    Heater stages
    Stage Incubator
    Sinks
    Small workbench

     

    Publication Acknowledgements:

    The facility should be listed in the "Acknowledgements" section of any publication using data generated in the OIF as follows:
      "Images were generated in the Core Optical Imaging Facility which is supported by UTHSCSA, NIH-NCI P30 CA54174 (CTRC at UTHSCSA) and NIH-NIA P01AG19316."