Abhik Bandyopadhyay, Ph.D.Assistant Professor/Research
University of Calcutta, India, 1986
I joined CSB as a research faculty in 2001. I obtained my Ph.D. degree in Biochemistry from Calcutta University on my studies in diabetes mellitus and hyperlipidemia at the Vivekananda Institute of Medical Sciences, Calcutta. My post-doctoral experience includes 1) cellular and molecular characterization of carcinogen induced mammary carcinoma utilizing mouse mammary gland organ culture model system at the Tumor Biology Laboratory in University of Nebraska, Lincoln, 2) drug induced Cytochrome P450 gene expression and regulation studies in tissue culture and rodent models and molecular mechanism of active drug transport into the mammary gland in rodent models at the department of Pharmacology and Experimental Therapeutics, College of Pharmacy, University of Kentucky, Lexington and 4) evaluating the role and targeting potential of transforming growth factor beta in advanced breast and prostate cancer in pre-clinical murine models at the department of Pharmacology of the University of Kentucky, Lexington.
My current research focuses on mammary stem cell biology studying the regulation of mammary stem cell function during aging and carcinogen exposure in relation to the development of age-associated breast cancer. Recent scientific evidence indicates that mammary stem cell lineages might be the cellular and molecular origin of certain types of breast cancer. But the knowledge of the cellular origin and molecular regulation of the breast cancer stem cells is still uncertain. The aged stem cells have been speculated to be more vulnerable to neoplastic transformation. As women over 50 years of age accounts for 75% of new breast cancer diagnosis, my research interest is to determine the intrinsic and extrinsic factors responsible for the altered function of mammary stem/progenitor cells during progressive aging leading to the generation of breast cancer stem cells.
Figure: Development of mouse mammary ductal and alveolar structures initiated by the mammary stem cells transplanted into the gland-free mammary fat pad. Formation of ducts at 8 weeks after transplantation (A) and full alveolar differentiation at pregnancy (B). Mammary stem cells were isolated from the mammary glands of a GFP transgenic mouse.
- Isolation and functional characterization of murine, non-human primate marmoset and human mammary stem and progenitor cells by flow-cytometry, mammosphere/3D-ECM/Colony forming cell assay and time-lapse imaging.
- In vivo mammary stem cell self-renewal/differentiation/transformation activity by mammary fat pad repopulation and serial propagation.
- Immunohistochemistry, immuno-fluorescence and whole mount staining, comet assay and RNA sequencing.
Bandyopadhyay A, Dong Q, Sun LZ. (2012) Stem/progenitor cells in murine mammary gland: isolation and functional characterization. Methods Mol Biol. 2012;879:179-93.
Bandyopadhyay A, Wang L, Agyin J, Tang Y, Lin S, Yeh IT, De K, Sun LZ. (2010) Doxorubicin in combination with a small TGFbeta inhibitor: a potential novel therapy for metastatic breast cancer in mouse models. PLoS One. 2010 Apr 28;5(4):e10365.
Bandyopadhyay A, Wang L, Chin SH, Sun LZ. (2007) Inhibition of skeletal metastasis by ectopic ERalpha expression in ERalpha-negative human breast cancer cell lines. Neoplasia. 2007 Feb;9(2):113-8.
Bandyopadhyay A, Agyin JK, Wang L, Tang Y, Lei X, Story BM, Cornell JE, Pollock BH, Mundy GR, Sun LZ. (2006) Inhibition of pulmonary and skeletal metastasis by a transforming growth factor-beta type I receptor kinase inhibitor. Cancer Res. 2006 Jul 1;66(13):6714-21.
Bandyopadhyay A, Wang L, Lόpez-Casillas F, Mendoza V, Yeh IT, Sun L. (2005) Systemic administration of a soluble betaglycan suppresses tumor growth, angiogenesis, and matrix metalloproteinase-9 expression in a human xenograft model of prostate cancer. Prostate. 2005 Apr 1;63(1):81-90.