Robert A. Marciniak, M.D., Ph.D.Associate Professor
We are currently utilizing a variety of approaches to probe the function of Werner protein at telomeres in primary and immortalized cells. Projects currently underway include: (1) The analysis of the interaction of WRN with telomere repeat binding factor 2 (TRF2) and the Fanconi anemia subgroup G protein. These proteins were identified as potential WRN interacting proteins utilizing a yeast two-hybrid screen. (2) The requirement for the function of the Werner protein for telomere maintenance is being tested through the construction of cell lines that contain an inducible, dominant-negative form of the protein. (3) The involvement of the Werner protein in DNA repair pathways is being studied using fluorescent protein reporter constructs.
Johnson F.B., Marciniak R.A., McVey M., Stewart S.A., Hahn W.C., and Guarente L. (2001) The S. cerevisiae WRN homologue Sgs1p participates in telomere maintenance in cells lacking telomerase. EMBO J 20:905-913.
Marciniak R.A., Lombard D.B., Johnson F.B. and Guarente L. (1998) Nucleolar localization of the Werner syndrome protein in human cells. Proc Natl Acad Sci USA 95:6887-6892.